![]() (C) 2000 Wiley- Liss, Inc.ĪB - A method for spatially three-dimensional (3D) localized two-dimensional (2D) 1H-13C correlation spectroscopy, localized HSQC, is proposed. The 3D localized 2D 1H-13C correlation spectra from a monkey brain in vivo were obtained after glucose injection, and amino acid metabolism was detected that is, glutamate appeared immediately after the injection, followed by the appearance of glutamate, glutamate, and glutamine. The localization capabilities of this method were confirmed in a phantom experiment. The preparation (echo) period 2τ can then be set substantially longer than 1/(2 1J(CH)), so that even in a whole-body system, slice-selective 90°(1H) pulses and gradient pulses can be applied in that period. The 180°(13C) and 180°(1H) pulses are separated in time, and the 180°(13C) pulse is applied at 1/(4 1J(CH)) before the 90°(1H) polarization transfer pulse. This method has the following special feature in the preparation period. N2 - A method for spatially three-dimensional (3D) localized two-dimensional (2D) 1H-13C correlation spectroscopy, localized HSQC, is proposed. T1 - 3D localized 1H-13C heteronuclear single-quantum coherence correlation spectroscopy in vivo (C) 2000 Wiley- Liss, Inc.Ībstract = "A method for spatially three-dimensional (3D) localized two-dimensional (2D) 1H-13C correlation spectroscopy, localized HSQC, is proposed. The 3D localized 2D 1H- 13C correlation spectra from a monkey brain in vivo were obtained after glucose injection, and amino acid metabolism was detected that is, glutamate appeared immediately after the injection, followed by the appearance of glutamate, glutamate, and glutamine. The preparation (echo) period 2τ can then be set substantially longer than 1/(2 1J(CH)), so that even in a whole-body system, slice-selective 90°( 1H) pulses and gradient pulses can be applied in that period. The 180°( 13C) and 180°( 1H) pulses are separated in time, and the 180°( 13C) pulse is applied at 1/(4 1J(CH)) before the 90°( 1H) polarization transfer pulse. In the delipidated heart FABP additional contacts between water molecules and NH protons could be observed using a three-dimensional rotating frame Overhauser 1H,15N heteronuclear single quantum coherence experiment obtained with a 15N-labeled sample of apo-heart FABP.A method for spatially three-dimensional (3D) localized two-dimensional (2D) 1H- 13C correlation spectroscopy, localized HSQC, is proposed. Most of the water molecules are localized in the gap between beta-strands D and E, and near the two alpha-helices. The spatial folding resembles a beta-barrel. The protein structure consists of 10 antiparallel beta-strands (betaA-betaJ), forming two nearly orthogonal beta-sheets, and a short helix-turn-helix motif connecting beta-strands A and B. Contacts between water protons and 23 NH protons of the protein backbone were identified. NOE and rotating-frame NOE (ROE) cross peaks between protein protons and protons of bound water molecules were observed in two-dimensional H2O-ROE/NOE-1H,15N-heteronuclear single quantum coherence spectra recorded from a uniformly 13C/15N-enriched sample of bovine heart FABP. Two- and three-dimensional heteronuclear NMR experiments have been performed to identify internally bound water molecules in the solution structure of bovine heart fatty-acid-binding protein (heart FABP). ![]()
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